Genomic Instability in Somatic Hybridization between Poncirus and Citrus Species Aiming to Create New Rootstocks

Rootstocks are an important component for citrus adaptation to increasing biotic and abiotic stresses resulting from global climate change. There is a strong complementarity between Citrus species, which adapt stresses, Poncirus trifoliata its intergeneric hybrids, exhibit resistances or tolerances major diseases pests. Thus, symmetrical somatic hybridization complementary diploid rootstocks of these two genera appears be efficient way develop new tetraploid in order address the challenges industry. New hybrids were obtained by electrofusion protoplasts P. hybrids. Extensive characterization nuclear cytoplasmic genomes was performed genotyping-by-sequencing (GBS) analysis. This revealed cybrids Mitochondrial mostly inherited callus parent, but homologous recombination events observed one parental combination. Chloroplasts exhibited random uniparental inheritance. GBS local chromosomal instabilities all whole chromosome eliminations However, at genome level, addition both parents predominant displayed least trifoliate orange haplotype throughout genome.